Active Recombinant Mouse Car4 protein(Met1-Ser277), His-tagged

• Cat.No.: Car4-662M
• Product Overview: Recombinant Mouse Car4 (NP_031633.1) without the C-terminal propeptide (Met 1-Ser 277) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser277
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its esterase activity. The specific activity is >10 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse Car4 consists of 271 amino acids and has a calculated molecular mass of 31 kDa as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Car4 carbonic anhydrase 4 [ Mus musculus ]
• Official Symbol: Car4
• Synonyms: CAR4; carbonic anhydrase 4; CA IV; CA-IV; carbonic anhydrase IV; carbonate dehydratase IV; Ca4; AW456718
• Gene ID: 12351
• mRNA Refseq: NM_007607
• Protein Refseq: NP_031633