Recombinant Human C4B Protein, His-tagged

• Cat.No.: C4B-343H
• Product Overview: Recombinant Human C4B Protein (Leu958-Gly1335) with N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes the basic form of complement factor 4, and together with the C4A gene, is part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 44 kDa
  Accurate Molecular Mass: 44 kDa
• Protein length: Leu958-Gly1335
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: C4B complement C4B (Chido blood group) [ Homo sapiens (human) ]
• Official Symbol: C4B
• Synonyms: C4B; complement C4B (Chido blood group); CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3; complement C4-B; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; Chido form of C4; basic complement C4; complement C4B1a; complement component 4B (Chido blood group)
• Gene ID: 721
• mRNA Refseq: NM_001002029
• Protein Refseq: NP_001002029
• MIM: 120820
• UniProt ID: P0C0L5