Recombinant Human C16orf70 293 Cell Lysate

Description :	Antigen standard for chromosome 16 open reading frame 70 (C16orf70) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	C16orf70 chromosome 16 open reading frame 70 [ Homo sapiens ]
Official Symbol	C16orf70
Synonyms	C16ORF70; chromosome 16 open reading frame 70; C16orf6, chromosome 16 open reading frame 6 , LIN10; UPF0183 protein C16orf70; FLJ12076; lin 10; lin-10 homolog; LIN10; lin-10; C16orf6;
Gene ID	80262
mRNA Refseq	NM_025187
Protein Refseq	NP_079463
UniProt ID	Q9BSU1
Chromosome Location	16q22.1
Function	glutamate receptor binding; protein binding;