Recombinant Human C12orf65 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: C12orf65-6454H
• Product Overview: C12orf65 MS Standard C13 and N15-labeled recombinant protein (NP_689482) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This nuclear gene encodes a mitochondrial matrix protein that appears to contribute to peptide chain termination in the mitochondrial translation machinery. Two different 1 bp deletions (resulting in the same premature stop codon)result in decreased mitochondrial translation, decreased levels of oxidative phosphorylation complexes and encepthalomyopathy. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 18.8 kDa
• AA Sequence: MSTVGLFHFPTPLTRICPAPWGLRLWEKLTLLSPGIAVTPVQMAGKKDYPALLSLDENELEEQFVKGHGPGGQATNKTSNCVVLKHIPSGIVVKCHQTRSVDQNRKLARKILQEKVDVFYNGENSPVHKEKREAAKKKQERKKRAKETLEKKKLLKELWESSKKVHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: C12orf65 chromosome 12 open reading frame 65 [ Homo sapiens (human) ]
• Official Symbol: C12orf65
• Synonyms: C12orf65; chromosome 12 open reading frame 65; CL065_HUMAN; COXPD7; mitochondrial; Probable peptide chain release factor C12orf65; probable peptide chain release factor C12orf65, mitochondrial; SPG55
• Gene ID: 91574
• mRNA Refseq: NM_152269
• Protein Refseq: NP_689482
• MIM: 613541
• UniProt ID: Q9H3J6