Recombinant Human BTLA protein, His-Avi-tagged, Biotinylated

• Cat.No.: BTLA-568H
• Product Overview: Biotinylated Recombinant Human BTLA protein(Q7Z6A9-1)(Lys31-Ser150), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Lys31-Ser150
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-BTLA Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: BTLA B and T lymphocyte associated [ Homo sapiens ]
• Official Symbol: BTLA
• Synonyms: BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; BTLA1; CD272; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein; FLJ16065; MGC129743
• Gene ID: 151888
• mRNA Refseq: NM_001085357
• Protein Refseq: NP_001078826
• MIM: 607925
• UniProt ID: Q7Z6A9