Recombinant Human BTD Protein (Ala42-Ala271), His tagged

• Cat.No.: BTD-0763H
• Product Overview: Recombinant human BTD (Ala42-Ala271) fused with His tag was expressed in E. coli.

Specification

• Description: Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing offats,carbohydrates, andproteins. Biotin is attached to these carboxylase enzymes through anamino acid (the building material of proteins) calledlysine, forming a complex calledbiocytin.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 27.17 kDa
• Protein length: Ala42-Ala271
• Purity: >90% as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles.
  Store at 2 to 8 centigrade for one week.
  Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS pH 7.5 or lyophilized from PBS pH 7.5.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: BTD biotinidase [ Homo sapiens (human) ]
• Official Symbol: BTD
• Synonyms: BTD; biotinidase; biotinase
• Gene ID: 686
• mRNA Refseq: NM_000060
• Protein Refseq: NP_000051
• MIM: 609019
• UniProt ID: P43251