Recombinant Human BRD4 protein, His&FLAG-tagged

• Cat.No.: BRD4-4829H
• Product Overview: Recombinant Human BRD4 protein(accession number NP_490597.1)(44-460 aa) was expressed in E. coli cells and contains an N-terminal His tag and a C-terminal FLAG tag.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His&C-FLAG
• Protein length: 44-460 aa
• Form: Supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 10% glycerol, 0.5 mM TCEP.
• Bio-activity: 1 μM H4K5/8/12/16(4ac) peptide was incubated with different concentrations of BRD4 (44-460) protein in 10 μl reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 μl Anti-FLAG antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.
• Molecular Mass: 51.6 kDa
• Purity: > 85%
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BRD4 bromodomain containing 4 [ Homo sapiens ]
• Official Symbol: BRD4
• Synonyms: BRD4; bromodomain containing 4; bromodomain-containing protein 4; CAP; chromosome associated protein; HUNK1; HUNKI; MCAP; bromodomain-containing 4; chromosome-associated protein
• Gene ID: 23476
• mRNA Refseq: NM_014299
• Protein Refseq: NP_055114
• MIM: 608749
• UniProt ID: O60885