Recombinant Human BRD4 protein, His&FLAG-tagged

• Cat.No.: BRD4-4831H
• Product Overview: Recombinant Human BRD4 protein(accession number NP_490597.1)(333-460 aa) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His&C-FLAG
• Protein length: 333-460 aa
• Form: Supplied in 25 mM Tris pH 7.5, 150 mM NaCl, 5% glycerol.
• Bio-activity: 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRD4 (333-460) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.
• Molecular Mass: 20.9 kDa
• Purity: >87% pure by SDS-PAGE.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BRD4 bromodomain containing 4 [ Homo sapiens ]
• Official Symbol: BRD4
• Synonyms: BRD4; bromodomain containing 4; bromodomain-containing protein 4; CAP; chromosome associated protein; HUNK1; HUNKI; MCAP; bromodomain-containing 4; chromosome-associated protein
• Gene ID: 23476
• mRNA Refseq: NM_014299
• Protein Refseq: NP_055114
• MIM: 608749
• UniProt ID: O60885