Active Recombinant Human BRD4 (BD2) Protein (342-460), N-His tagged

• Cat.No.: BRD4-38H
• Product Overview: Active Recombinant human BRD4 (BD2) (342-460) was expressed in E. coli cells using an N-terminal His tag.

Specification

• Description: Bromodomain-containing protein 4 (BRD4) is a member of the bromodomain and extra-terminal domain (BET) protein family. BRD4 contains two tandem bromodomains: BD1 and BD2. BRD4 (BD2) interacts with acetylated histones and other transcription factors, influencing the assembly and stability of large transcriptional complexes at specific genomic loci. It serves as a scaffold for the recruitment of various coregulatory proteins, contributing to the activation or repression of target genes. BRD4 (BD2) plays a critical role in cell cycle progression, as well as the maintenance of pluripotency and self-renewal in embryonic stem cells.
• Source: E. coli
• Species: Human
• Tag: His
• Bio-activity: 531 ng/min/mg
• Molecular Mass: Observed MW: ~14 kDa
  Calculated MW: ~14.3 kDa
• Protein length: 342-460
• Purity: > 90%
• Stability: One year at –70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: BRD4 bromodomain containing 4 [ Homo sapiens (human) ]
• Official Symbol: BRD4
• Synonyms: BRD4; bromodomain containing 4; bromodomain-containing protein 4; CAP; chromosome associated protein; HUNK1; HUNKI; MCAP; bromodomain-containing 4; chromosome-associated protein
• Gene ID: 23476
• mRNA Refseq: NM_014299
• Protein Refseq: NP_055114
• MIM: 608749
• UniProt ID: O60885