Recombinant Human BRD2 protein, His&FLAG-tagged

• Cat.No.: BRD2-4347H
• Product Overview: Recombinant Human BRD2 protein (NM_005104.3)(344-455 aa) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His&C-FLAG
• Protein length: 344-455 aa
• Form: Supplied in 25 mM Tris pH 8.0, 150 mM NaCl, 10% glycerol, 1 mM TCEP.
• Bio-activity: 3 μM histone peptide H4K5/8/12/16(4Ac) was incubated with BRD2 (344-455) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.
• Molecular Mass: 19.4 kDa
• Purity: >90% pure by SDS-PAGE
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BRD2 bromodomain containing 2 [ Homo sapiens ]
• Official Symbol: BRD2
• Synonyms: BRD2; bromodomain containing 2; bromodomain-containing protein 2; D6S113E; FSRG1; KIAA9001; NAT; RING3; O27.1.1; bromodomain-containing 2; really interesting new gene 3 protein; female sterile homeotic-related gene 1; FSH; RNF3; FLJ31942; DKFZp686N0336
• Gene ID: 6046
• mRNA Refseq: NM_001113182
• Protein Refseq: NP_001106653
• MIM: 601540
• UniProt ID: P25440