Recombinant Human BRCA1 protein, His-tagged

• Cat.No.: BRCA1-6881H
• Product Overview: Recombinant Human BRCA1 aa. (Gln19~Val83 (Accession # P38398)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 11kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gln19~Val83
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: BRCA1 BRCA1, DNA repair associated [ Homo sapiens (human) ]
• Official Symbol: BRCA1
• Synonyms: IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53; BRCA1/BRCA2-containing complex, subunit 1; Fanconi anemia,; complementation group S; RING finger protein 53; breast and ovarian cancer susceptibility protein 1; breast cancer 1, early onset; early onset breast cancer 1; protein phosphatase 1, regulatory subunit 53; breast cancer type 1 susceptibility protein
• Gene ID: 672
• mRNA Refseq: NM_007294.3
• Protein Refseq: NP_009225.1
• UniProt ID: P38398