Recombinant Human BMP4, His-tagged

• Cat.No.: BMP4-21H
• Product Overview: Recombinant Human BMP4 encoding the mature human bone morphogenetic protein 4 (Ser293-Arg408) was recombined with Hexa-histidine tag at the N-Terminus. That was expressed inE.Coli,and purified by Ni-NTA column. MW = 16 KD.

Specification

• Cat. No.: BMP4 - 21H
• Description: Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva.
• Source: Escherichia Coli.
• Amino Acid Sequence: human bone morphogenetic protein 4 (Ser293-Arg408). 293 SPKHHSQRAR KKNKNCRRHS LYVDFSDVGWQAFYCHGDCP FPLADHLNST NHAIVQTLVNCVP TELSAIS MLYLDEYDKV VLKNYQEMVVGW NDWIVAPPGYVN SVNSSIPKACVV EGCGCR 408.
• Application: Cell biology.
• Purity: >95% in SDS gel.
• Formulation: Lyophilized 100 μg human bone morphogenetic protein-8B in 50 μl of TBS ( 20 mM Tris, 50mM NaCl,pH8.0 ). Carry free.
• Reconstitution: Add 500 μl deionized water to the vial to prepare a working stock solution at 200 μg/mL . Allow to set at least 30 minutes at 4° C, mix well.
• Storage: Store lyophilized protein at -20 °C or -70 °C. Lyophilized protein is stable for up to 6 months from date of receipt at - 20 °C to -70 °C. Upon reconstitution, this protein can be stored at -20 °C for a few weeks or at -70 °C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
• Protein length: 293-408 a.a.

Gene Information

• Gene Name: BMP4 bone morphogenetic protein 4 [ Homo sapiens ]
• Synonyms: ZYME; BMP2B; BMP2B1; DVR4; MCOPS6; BMP-2B; BMP-4; Bone morphogenetic protein 4 precursor; bone morphogenetic protein 2B; bone morphogenetic protein 4; BMP4
• Gene ID: 652
• mRNA Refseq: NM_001202
• Protein Refseq: NP_001193
• MIM: 112262
• UniProt ID: P12644
• Chromosome Location: 14q22-q23
• Function: cytokine activity. growth factor activity. heparin binding. protein binding. signal transducer activity