Recombinant Human BID

• Cat.No.: BID-26711TH
• Product Overview: Recombinant full length human Bid with N-terminal proprietary tag expressed in a Baculovirus infected Sf9 cell expression system; amino acids 1-95, 52kDa.

Specification

• Description: This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
• Tissue specificity: Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is
• Form: Liquid
• Storage buffer: Preservative: 150mM ImidazoleConstituents: 25% Glycerol, 50mM MOPS, 150mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Tag: Non
• Protein length: 1-95

Gene Information

• Gene Name: BID BH3 interacting domain death agonist [ Homo sapiens ]
• Official Symbol: BID
• Synonyms: BID; BH3 interacting domain death agonist; BH3-interacting domain death agonist
• Gene ID: 637
• mRNA Refseq: NM_001196
• Protein Refseq: NP_001187
• MIM: 601997
• Uniprot ID: P55957
• Chromosome Location: 22q11.2
• Pathway: Activation and oligomerization of BAK protein, organism-specific biosystem; Activation of BAD and translocation to mitochondria, organism-specific biosystem; Activation of BH3-only proteins, organism-specific biosystem; Activation, myristolyation of BID and translocation to mitochondria, organism-specific biosystem; Activation, translocation and oligomerization of BAX, organism-specific biosystem
• Function: death receptor binding; protein binding; ubiquitin protein ligase binding

Reviews

12/19/2021

Activity retention time is very long and can be maintained for a long time.

09/13/2021

The detection method is very sensitive and can easily detect small amounts of protein.

04/10/2021

Expression level and stability are good and will be repurchased.

Q&As

In which cellular processes does the BID protein play?

BID protein mainly plays a role in the process of apoptosis. It can regulate cell survival and death signaling by interacting with other members of the Bcl-2 family.

Can the expression level and activity of BID protein be determined?

The expression level of BID protein can be detected by immunohistochemistry, western blotting, real-time quantitative PCR and other techniques. At the same time, the activity of BID protein can be assessed by detecting the level of phosphorylation of BID protein and the number of BID protein within cells.

How are BID proteins involved in the process of apoptosis?

During apoptosis, the BID protein is activated, forming complexes with other Bcl-2 family members, releasing cytochrome c, initiating a cascade of caspase enzymes, and ultimately leading to apoptosis.

How are the interactions between BID proteins and other proteins regulated?

The interaction between BID protein and other proteins is regulated by a variety of factors, including phosphorylation, methylation, acetylation and other modifications, as well as interactions with other proteins. These factors can affect the interaction between BID protein and other proteins, which in turn affects the regulatory process of apoptosis.

How can BID proteins be used as biomarkers in clinical practice?

In clinical practice, the expression level of BID protein can be measured to predict disease progression and prognosis, and to guide the choice of treatment regimen. For example, in some tumor diseases, measuring the expression level of BID protein can help doctors determine the type, stage, and prognosis of the disease, and guide the choice of treatment regimen.

Can the expression level of BID protein be regulated?

Expression level of BID protein is regulated by a variety of factors, including gene transcription, translation, modification, and degradation. These factors can affect the expression level of BID protein, which in turn affects the survival and death signals of cells.