Recombinant Human Bcl-2 BH4 [1-218, BH4(10-30)]

• Cat.No.: BCL2-147H
• Product Overview: Recombinant Human Bcl-2 produced in E.Coli is a single, non-glycosylated polypeptide chain. The rHuBcl-2 is expressed as GST fusion protein and purified by proprietary romatographic techniques, after purification, the GST tag can be removed.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: Bcl-2 is the prototype for a family of mammalian genes and the proteins they produce. They govern mitochondrial outer membrane permeabilization (MOMP) and can be either pro-apoptotic (Bax, BAD, Bak and Bok among others) or anti-apoptotic (including Bcl-2 proper, Bcl-xL, and Bcl-w, among an assortment of others). There are a total of 25 genes in the Bcl-2 family known to date. Bcl-2 derives its name from B-cell lymphoma 2, as it is the second member of a range of proteins initially described as a reciprocal gene translocation in chromosomes 14 and 18 in follicular lymphomas.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Formulation: The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
• Solubility: It is recommended to reconstitute the lyophilized rHuBcl-2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuBcl-2.
• Applications: Input marker or positive control (Western Blotting). Function study (binding assay).
• Storage: Lyophilized rHuBcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution rHuInsulin should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Synonyms: Bcl-2; OTTHUMP00000163680; BCL2; B-cell CLL/lymphoma 2; B-cell lymphoma protein 2.