Recombinant Human B-cell CLL/lymphoma 2, His-tagged

• Cat.No.: BCL2-509H
• Product Overview: Recombinant Bcl2 protein was expressed inE.coliand purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. MW = 25.4 kDa (231 aa).

Specification

• Cat. No.: BCL2-509H
• Description: Bcl2, also known as B-cell lymphoma protein 2 alpha, is an anti-apoptotic protein located primarily in the outer mitochondrial membrane that blocks the apoptotic death of some cells such as lymphocytes. BCL2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apotosis. Bcl2 exerts its anti-apoptotic function through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). The Bcl2 gene has been related with a number of cancers, including melanoma, breast, prostate, and lung carcinomas, as well as schizophrenia and autoimmunity.
• Source: E.coli.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE LYGPSMRPLFD.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol 2 Mm DTT.
• Purity: > 90% by SDS – PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: BCL2 B-cell CLL/lymphoma 2 [ Homo sapiens ]
• Synonyms: Bcl-2; OTTHUMP00000163680; BCL2; B-cell CLL/lymphoma 2; B-cell lymphoma protein 2
• Gene ID: 596
• mRNA Refseq: NM_000633
• Protein Refseq: NP_000624
• MIM: 151430
• UniProt ID: P10415
• Chromosome Location: 18q21.33; 18q21.3
• Pathway: Amyotrophic lateral sclerosis (ALS); Apoptosis; Colorectal cancer; Focal adhesion; Neurotrophin signaling pathway; Pathways in cancer; Prostate cancer; Small cell lung cancer
• Function: BH3 domain binding; Protease binding; Protein Heterodimerization activity; Protein homodimerization activity; Transcription activator activity; Transcription factor binding