Recombinant Human Aurora Kinase B, GST-His

• Cat.No.: AURKB-684H
• Product Overview: Recombinant human Aurora B kinase consisting of mino acids 2-344 was fused at the N-terminus to a GST-His Tag sequence. It was expressed inS. frugiperdainsect cells using a baculovirus expression system.MW = 70000 Da.

Specification

• Cat. No.: AURKB-684H
• Description: Aurora B is a mitotic kinase that phosphorylates histone H3 and CenpA. Upregulation of Aurora B is reported in pancreatic and prostate cancers. The active kinase is useful in the study of Aurora B regulation and for inhibitor screening.
• Source/Host: S. frugiperda.
• Form: Liquid. In 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 detergent, pH 8.0. Please refer to vial label for lot-specific concentration.
• Specific Activity: ≥12 units/µg protein.
• Purity: ≥70% by SDS-PAGE.
• Unit Definition: One unit is defined as the amount of enzyme that is required to phosphorylate 1 pmol Ser/Thr 1 peptide substrate per min at 30°C, pH 7.4.
• Toxicity: Standard Handling (MSDS available upon request).
• Storage: ≤ -70°C. Avoid freeze/thaw Following intitial thaw, aliquot and freeze (-70°C).
• Protein length: 2-344 a.a.

Gene Information

• Gene Name: AURKB aurora kinase B [ Homo sapiens ]
• Synonyms: AURKB; aurora kinase B ; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; aurkb-sv1; aurkb-sv2; aurora-1; aurora-B; aurora kinase B-Sv1; aurora kinase B-Sv2; serine/threonine kinase 12; EC 2.7.11.1; Aurora- and Ipl1-like midbody-associated protein 1; Aurora-related kinase 2; Aurora/IPL1-related kinase 2
• Gene ID: 9212
• mRNA Refseq: NM_004217
• Protein Refseq: NP_004208
• MIM: 604970
• UniProt ID: Q96GD4
• Chromosome Location: 17p13.1
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity