Recombinant Human ATP7B Protein, His tagged

• Cat.No.: ATP7B-02H
• Product Overview: Purified recombinant protein of Human ATPase, Cu++ transporting, beta polypeptide (ATP7B), transcript variant 1, 200His-482Thr, with N-terminal His tag, expressed in E.coli.

Specification

• Description: This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein is a monomer, and functions as a copper-transporting ATPase which exports copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease which is characterized by copper accumulation.
• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: 200-482 aa
• Molecular Mass: 31.9kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Storage Buffer: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 10% glycerol
• Concentration: > 0.05 μg/μL as determined by microplate BCA method

Gene Information

• Gene Name: ATP7B ATPase copper transporting beta [ Homo sapiens (human) ]
• Official Symbol: ATP7B
• Synonyms: ATP7B; ATPase copper transporting beta; WD; PWD; WC1; WND; copper-transporting ATPase 2; ATPase, Cu(2+)- transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide; Wilson disease-associated protein; copper pump 2; copper-transporting protein ATP7B; EC 7.2.2.8
• Gene ID: 540
• mRNA Refseq: NM_000053
• Protein Refseq: NP_000044
• MIM: 606882
• UniProt ID: P35670