Recombinant Human ATG10, GST-tagged

• Cat.No.: ATG10-9968H
• Product Overview: Recombinant Human ATG10 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: 1-220a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: ATG10 ATG10 autophagy related 10 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ATG10
• Synonyms: ATG10; ATG10 autophagy related 10 homolog (S. cerevisiae); APG10 autophagy 10 like (S. cerevisiae) , APG10L; ubiquitin-like-conjugating enzyme ATG10; DKFZP586I0418; FLJ13954; autophagy-related protein 10; APG10; APG10L; pp12616; DKFZp586I0418
• Gene ID: 83734
• mRNA Refseq: NM_001131028
• Protein Refseq: NP_001124500
• MIM: 610800
• UniProt ID: Q9H0Y0
• Chromosome Location: 5q14.1
• Pathway: Senescence and Autophagy, organism-specific biosystem
• Function: Atg12 ligase activity; ligase activity