Recombinant Human ATAD2, His&FLAG-tagged

• Cat.No.: ASH1L-30H
• Product Overview: Recombinant Human ASH1L corresponding to amino acids 2407-2579 that contains the bromodomain sequences of ASH1L (accession number NP_060959.2) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag.

Specification

• Description: ASH1L is a member of the Trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. The bromodomain functions as a "reader" of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. ASH1L is localized to the nucleus and also to cell-cell tight junctions where it is hypothesized to function in adhesion-mediated signaling. ASH1L functions in methylation of H3K4 and regulation of expression of HOX genes and may possess methyltransferase activity directed at H3K36. ASH1L has also been implicated in facioscapulohumeral muscular dystrophy.
• Source: E. coli
• Species: Human
• Tag: His&Flag
• Form: 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.
• Molecular Mass: 25.6 kDa
• Applications: Binding assays, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 6.7 mg/ml
• Protein length: 2407-2579 a.a.

Gene Information

• Gene Name: ASH1L ash1 (absent, small, or homeotic)-like (Drosophila) [ Homo sapiens ]
• Official Symbol: ASH1L
• Synonyms: ASH1L; ash1 (absent, small, or homeotic)-like (Drosophila); histone-lysine N-methyltransferase ASH1L; ASH1; ASH1L1; huASH1; KMT2H; ASH1-like protein; lysine N-methyltransferase 2H; absent small and homeotic disks protein 1 homolog; probable histone-lysine N-methyltransferase ASH1L; FLJ10504; KIAA1420
• Gene ID: 55870
• mRNA Refseq: NM_018489
• Protein Refseq: NP_060959
• MIM: 607999
• UniProt ID: Q9NR48
• Chromosome Location: 1q22
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; Tight junction, organism-specific biosystem; Tight junction, conserved biosystem
• Function: DNA binding; histone-lysine N-methyltransferase activity; metal ion binding; methyltransferase activity; transferase activity; zinc ion binding