Recombinant Human ARPC2 293 Cell Lysate

• Cat.No.: ARPC2-8685HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for actin related protein 2/3 complex, subunit 2, 34kDa (ARPC2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ARPC2 actin related protein 2/3 complex, subunit 2, 34kDa [ Homo sapiens ]
• Official Symbol: ARPC2
• Synonyms: ARPC2; actin related protein 2/3 complex, subunit 2, 34kDa; actin related protein 2/3 complex, subunit 2 (34 kD); actin-related protein 2/3 complex subunit 2; ARC34; p34 Arc; arp2/3 complex 34 kDa subunit; ARP2/3 protein complex subunit 34; PRO2446; p34-Arc; PNAS-139
• Gene ID: 10109
• mRNA Refseq: NM_005731
• Protein Refseq: NP_005722
• MIM: 604224
• UniProt ID: O15144
• Chromosome Location: 2q36.1
• Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; CDC42 signaling events, organism-specific biosystem; ErbB1 downstream signaling, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem
• Function: actin binding; protein binding; structural constituent of cytoskeleton

Q&As

Can you provide a characterization of your recombinant ARPC2 protein? Is the recombinant ARPC2 protein functional? What analysis have do you as part of the protein characterization?

The recombinant ARPC2 protein is a modified version of the natural ARPC2 protein, produced through genetic engineering techniques. ARPC2 is a subunit of the ARP2/3 protein complex, which plays a critical role in regulating actin cytoskeletal dynamics. The recombinant ARPC2 protein is typically designed for research purposes and is often used as a tool to study the function and interactions of the ARP2/3 complex. It is generated by introducing the genetic sequence of ARPC2 into a suitable expression system, such as bacteria, yeast, or mammalian cells, that can produce large quantities of the protein. The recombinant ARPC2 protein possesses similar structural and functional characteristics as the native ARPC2 protein. It typically retains the ability to interact with other subunits of the ARP2/3 complex and facilitates the formation of branched actin networks. This essential property allows researchers to investigate the mechanisms underlying actin polymerization and cytoskeletal remodeling in various biological processes. Moreover, the recombinant ARPC2 protein can be tagged with specific markers, such as fluorescent tags or affinity tags, to enable easy detection, purification, and visualization. This versatility enhances the utility of the protein in experimental studies. Overall, the recombinant ARPC2 protein serves as a valuable tool for understanding the cellular processes governed by the ARP2/3 complex and provides insights into its involvement in various physiological and pathological conditions. Our routine QC of ARPC2 protein includes BCA, SDS-PAGE, Western Blot, and Endotoxin detection. Here below are the optional services, case to case. Activity Test; HPLC; SEC; Protein Biotinylation; Protein labeling/conjugation.