Recombinant Human Arginase, Liver, His-tagged

• Cat.No.: ARG1-2489H
• Product Overview: Recombinant human ARG1, fused to His-tag at C-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 35.8kDa (330aa).

Specification

• Cat. No.: ARG1-2489H
• Description: Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The type II isoform has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate.
• Source: E.coli.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl.
• Purity: > 85% by SDS – PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GD LP F ADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSL AIGS IS G HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK EL KGKIPDVP GF SWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEV DR LGIGK VME ET LSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TE EIYKTGLL S GLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NH KP IDYLNP PKL EH HHHHH.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ARG1 arginase, liver [ Homo sapiens ]
• Synonyms: arginase, liver; ARG1; arginase-1; arginase 1; type I arginase; liver-type arginase; EC 3.5.3.1; Type I arginase; Liver-type arginase
• Gene ID: 383
• mRNA Refseq: NM_000045
• Protein Refseq: NP_000036
• MIM: 608313
• UniProt ID: P05089
• Chromosome Location: 6q23
• Pathway: Arginine and proline metabolism; Metabolic pathways; Metabolism of amino acids and derivatives
• Function: arginase activity; hydrolase activity; metal ion binding