Recombinant Human AREG protein, hFc-tagged

• Cat.No.: AREG-754H
• Product Overview: Recombinant Human AREG protein(P15514)(Ser101-Lys187), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N-hFc
• Protein length: Ser101-Lys187
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 37.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: AREG amphiregulin [ Homo sapiens ]
• Official Symbol: AREG
• Synonyms: AREG; amphiregulin; schwannoma derived growth factor , SDGF; schwannoma-derived growth factor; colorectum cell-derived growth factor; AR; SDGF; AREGB; CRDGF; MGC13647
• Gene ID: 374
• mRNA Refseq: NM_001657
• Protein Refseq: NP_001648
• MIM: 104640
• UniProt ID: P15514