Recombinant Human APRT protein, His-tagged

• Cat.No.: APRT-591H
• Product Overview: Recombinant Human APRT (P07741) aa. (Met1~Glu180) fused with His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1~Glu180
• Description: Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23.3kDa; Accurate Molecular Mass: 23kDa as determined by SDS-PAGE reducing conditions.
• Purity: >98%
• Characteristic: Isoelectric point is 6.8.
• Applications: Cell culture; Activity Assays; In vivo assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: APRT adenine phosphoribosyltransferase [ Homo sapiens (human) ]
• Official Symbol: APRT
• Synonyms: APRT; adenine phosphoribosyltransferase; AMP; APRTD; AMP diphosphorylase; AMP pyrophosphorylase; transphosphoribosidase
• Gene ID: 353
• mRNA Refseq: NM_000485.2
• Protein Refseq: NP_000476.1
• UniProt ID: P07741