Recombinant Human APOE protein, His-tagged, Biotinylated(Primary Amine Labeling)

• Cat.No.: APOE-4674H
• Product Overview: Biotinylated Recombinant Human APOE protein(P02649-1)(Lys19-His317)(Primary Amine Labeling), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Lys19-His317
• Tag: N-His
• Conjugation/Label: Biotin; Primary Amine
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human APOE3, His Tag with an affinity constant of 1.41 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: APOE apolipoprotein E [ Homo sapiens ]
• Official Symbol: APOE
• Synonyms: APOE; apolipoprotein E; AD2, Alzheimer disease 2 (APOE*E4 associated, late onset); apo-E; apolipoprotein E3; AD2; LPG; LDLCQ5; MGC1571
• Gene ID: 348
• mRNA Refseq: NM_000041
• Protein Refseq: NP_000032
• UniProt ID: P02649