Recombinant Human APOE protein, His-tagged

• Cat.No.: APOE-57H
• Product Overview: Recombinant Human APOE protein(P02649-1)(Lys19-His317), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Lys19-His317
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE3, His Tag with an affinity constant of 1.21 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: APOE apolipoprotein E [ Homo sapiens ]
• Official Symbol: APOE
• Synonyms: APOE; apolipoprotein E; AD2, Alzheimer disease 2 (APOE*E4 associated, late onset); apo-E; apolipoprotein E3; AD2; LPG; LDLCQ5; MGC1571
• Gene ID: 348
• mRNA Refseq: NM_000041
• Protein Refseq: NP_000032
• UniProt ID: P02649