Recombinant Human APLN protein, hFc-tagged

• Cat.No.: APLN-7854H
• Product Overview: Recombinant Human APLN protein(Q9ULZ1)(Gly23-Phe77), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-hFc
• Protein length: Gly23-Phe77
• Form: Supplied as 0.22μm filtered solution in PBS, 200mM Arginine (pH 7.4).
• Bio-activity: Immobilized Human APLN, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 42.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: APLN apelin [ Homo sapiens ]
• Official Symbol: APLN
• Synonyms: APLN; apelin; apelin, AGTRL1 ligand; XNPEP2; AGTRL1 ligand; APJ endogenous ligand; APEL
• Gene ID: 8862
• mRNA Refseq: NM_017413
• Protein Refseq: NP_059109
• MIM: 300297
• UniProt ID: Q9ULZ1