Recombinant Human API5 protein, His-tagged

• Cat.No.: API5-1171H
• Product Overview: Recombinant Human API5 aa. (Pro2~Phe360 fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Pro2~Phe360
• Description: This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
• Form: Freeze-dried powder
• Molecular Mass: 44kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >97%
• Characteristic: The isoelectric point is 4.7.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: API5 apoptosis inhibitor 5 [ Homo sapiens (human) ]
• Official Symbol: API5
• Synonyms: API5; apoptosis inhibitor 5; AAC11; AAC-11; FIF; antiapoptosis clone 11 protein; cell migration-inducing gene 8 protein; fibroblast growth factor 2-interacting factor 2; migration-inducing protein MIG8
• Gene ID: 8539
• mRNA Refseq: NM_001142930.1
• Protein Refseq: NP_001136402.1
• UniProt ID: Q9BZZ5