Recombinant Human ALPI protein, His-Flag-tagged
| Cat.No. : | ALPI-4636H |
| Product Overview : | Recombinant Human ALPI protein(P09923)(Val20-Asp503), fused with N-terminal His-Flag tag, was expressed in HEK293. |
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| Source : | HEK293 |
| Species : | Human |
| Tag : | N-His-Flag |
| Protein length : | Val20-Asp503 |
| Form : | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Bio-activity : | Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline phosphatase (Intestinal type) , His Tag with an affinity constant of 1.58 μM as determined in SPR assay (Biacore T200). |
| Molecular Mass : | The protein has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result. |
| Endotoxin : | Less than 1EU per μg by the LAL method. |
| Purity : | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Storage : | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Reconstitution : | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Gene Name | ALPI alkaline phosphatase, intestinal [ Homo sapiens ] |
| Official Symbol | ALPI |
| Synonyms | ALPI; alkaline phosphatase, intestinal; intestinal-type alkaline phosphatase; Kasahara isozyme; glycerophosphatase; alkaline phosphomonoesterase; intestinal alkaline phosphatase; IAP; |
| Gene ID | 248 |
| mRNA Refseq | NM_001631 |
| Protein Refseq | NP_001622 |
| MIM | 171740 |
| UniProt ID | P09923 |
Not For Human Consumption!
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