Active Recombinant Human ALPI Protein, Fc-tagged
| Cat.No. : | ALPI-513H |
| Product Overview : | Recombinant mature form of human ALPI (P09923) (Met 1-Asp 503),without the pro peptide, was fused with a polyhistidine tag at the C-terminus. |
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| Source : | HEK293 |
| Species : | Human |
| Tag : | Fc |
| Predicted N Terminal : | Val 20 |
| Form : | Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol. |
| Bio-activity : | Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).The specific activity is >10,000 pmoles/min/μg. |
| Molecular Mass : | The secreted recombinant human ALPI/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 725 amino acids and has a predicted molecular mass of 79.5 kDa. The apparent molecular mass of rhALPI /Fc monomer is approximately 90-95 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : | >83 % as determined by SDS-PAGE. |
| Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Storage : | Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
| Protein length : | 1-503 a.a. |
| Gene Name | ALPI alkaline phosphatase, intestinal [ Homo sapiens ] |
| Official Symbol | ALPI |
| Synonyms | ALPI; alkaline phosphatase, intestinal; intestinal-type alkaline phosphatase; Kasahara isozyme; glycerophosphatase; alkaline phosphomonoesterase; intestinal alkaline phosphatase; IAP; |
| Gene ID | 248 |
| mRNA Refseq | NM_001631 |
| Protein Refseq | NP_001622 |
| MIM | 171740 |
| UniProt ID | P09923 |
| Chromosome Location | 2q37.1 |
| Pathway | Folate biosynthesis, organism-specific biosystem; Folate biosynthesis, conserved biosystem; Metabolic pathways, organism-specific biosystem; |
Not For Human Consumption!
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