Recombinant Human ALKBH3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ALKBH3-5816H
• Product Overview: ALKBH3 MS Standard C13 and N15-labeled recombinant protein (NP_631917) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 33.2 kDa
• AA Sequence: MEEKRRRARVQGAWAAPVKSQAIAQPATTAKSHLHQKPGQTWKNKEHHLSDREFVFKEPQQVVRRAPEPRVIDREGVYEISLSPTGVSRVCLYPGFVDVKEADWILEQLCQDVPWKQRTGIKEDITYQQPRLTAWYGELPYTYSRITMEPNPHWHPVLRTLKNRIEENTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEMRKKPPPEENGEYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSREPRVNLTFRTVYPDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ALKBH3 alkB homolog 3, alpha-ketoglutarate dependent dioxygenase [ Homo sapiens (human) ]
• Official Symbol: ALKBH3
• Synonyms: ALKBH3; alkB, alkylation repair homolog 3 (E. coli); alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; DEPC 1; prostate cancer antigen 1; prostate cancer antigen-1; alkylated DNA repair protein alkB homolog 3; ABH3; PCA1; DEPC1; DEPC-1; FLJ43614; MGC118790; MGC118792; MGC118793
• Gene ID: 221120
• mRNA Refseq: NM_139178
• Protein Refseq: NP_631917
• MIM: 610603
• UniProt ID: Q96Q83