Recombinant Human ALKBH3 293 Cell Lysate

• Cat.No.: ALKBH3-8901HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for alkB, alkylation repair homolog 3 (E. coli) (ALKBH3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ALKBH3 alkB, alkylation repair homolog 3 (E. coli) [ Homo sapiens ]
• Official Symbol: ALKBH3
• Synonyms: ALKBH3; alkB, alkylation repair homolog 3 (E. coli); alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; DEPC 1; prostate cancer antigen 1; prostate cancer antigen-1; alkylated DNA repair protein alkB homolog 3; ABH3; PCA1; DEPC1; DEPC-1; FLJ43614; MGC118790; MGC118792; MGC118793
• Gene ID: 221120
• mRNA Refseq: NM_139178
• Protein Refseq: NP_631917
• MIM: 610603
• UniProt ID: Q96Q83
• Chromosome Location: 11p11.2
• Pathway: ABH3 mediated Reversal of Alkylation Damage, organism-specific biosystem; DNA Damage Reversal, organism-specific biosystem; DNA Repair, organism-specific biosystem; Reversal of Alkylation Damage By DNA Dioxygenases, organism-specific biosystem
• Function: DNA-N1-methyladenine dioxygenase activity; L-ascorbic acid binding; cytosine C-5 DNA demethylase activity; ferrous iron binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen