Recombinant Human ALDOB, His-tagged

• Cat.No.: ALDOB-9567H
• Product Overview: Recombinant Human ALDOB protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Description: Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related housekeeping genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.
• Source: E.coli
• Species: Human
• Tag: His
• Protein length: 1-316a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: ALDOB aldolase B, fructose-bisphosphate [ Homo sapiens ]
• Official Symbol: ALDOB
• Synonyms: ALDOB; aldolase B, fructose-bisphosphate; fructose-bisphosphate aldolase B; aldolase 2; liver-type aldolase; aldolase B, fructose-bisphosphatase; ALDB; ALDO2
• Gene ID: 229
• mRNA Refseq: NM_000035
• Protein Refseq: NP_000026
• MIM: 612724
• UniProt ID: P05062
• Chromosome Location: 9q21.3-q22.2
• Pathway: FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem; Fructose catabolism, organism-specific biosystem; Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, organism-specific biosystem
• Function: ATPase binding; cytoskeletal protein binding; fructose binding; fructose-bisphosphate aldolase activity; fructose-bisphosphate aldolase activity; identical protein binding; lyase activity; protein binding