Recombinant Human ALDH6A1, His-tagged

• Cat.No.: ALDH6A1-67H
• Product Overview: Recombinant human ALDH6A1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: ALDH6A1 also known as Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial. ALDH6A1 is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This protein catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. ALDH6A1 deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid, In Phosphate buffered saline (pH7.4) containing 10% glycerol, 1mM DTT
• Molecular Mass: 56.8kDa (525aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSSSSVPTV KLFIGGKFVE SKSDKWIDIH NPATNEVIGR VPQATKAEMD AAIASCKRAF PAWADTSVLS RQQVLLRYQQ LIKENLKEIA KLITLEQGKT LADAEGDVFR GLQVVEHACS VTSLMMGETM PSITKDMDLY SYRLPLGVCA GIAPFNFPAM IPLWMFPMAM VCGNTFLMKP SERVPGATML LAKLLQDSGA PDGTLNIIHG QHEAVNFICD HPDIKAISFV GSNKAGEYIF ERGSRHGKRV QANMGAKNHG VVMPDANKEN TLNQLVGAAF GAAGQRCMAL STAVLVGEAK KWLPELVEHA KNLRVNAGDQ PGADLGPLIT PQAKERVCNL IDSGTKEGAS ILLDGRKIKV KGYENGNFVG PTIISNVKPN MTCYKEEIFG PVLVVLETET LDEAIQIVNN NPYGNGTAIF TTNGATARKY AHLVDVGQVG VNVPIPVPLP MFSFTGSRSS FRGDTNFYGK QGIQFYTQLK TITSQWKEED ATLSSPAVVM PTMGR
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ALDH6A1 aldehyde dehydrogenase 6 family, member A1 [ Homo sapiens ]
• Official Symbol: ALDH6A1
• Synonyms: ALDH6A1; aldehyde dehydrogenase 6 family, member A1; MMSDH; methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial; malonate-semialdehyde dehydrogenase; aldehyde dehydrogenase family 6 member A1; mitochondrial acylating methylmalonate-semialdehyde dehydrogenase; MMSADHA; MGC40271
• Gene ID: 4329
• mRNA Refseq: NM_005589
• Protein Refseq: NP_005580
• MIM: 603178
• UniProt ID: Q02252
• Chromosome Location: 14q24
• Pathway: Branched-chain amino acid catabolism, organism-specific biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem; Malonate semialdehyde pathway, propanoyl-CoA =>Acetyl-CoA, organism-specific biosystem; Malonate semialdehyde pathway, propanoyl-CoA => Acetyl-CoA, conserved biosystem
• Function: fatty-acyl-CoA binding; malonate-semialdehyde dehydrogenase (acetylating) activity; methylmalonate-semialdehyde dehydrogenase (acylating) activity; oxidoreductase activity