Recombinant Human AKAP12 protein, His-tagged

• Cat.No.: AKAP12-8983H
• Product Overview: Recombinant Human AKAP12(Pro1485~Ser1782) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300
• Molecular Mass: 36.0kDa
• Protein length: Pro1485~Ser1782
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: AKAP12 A kinase (PRKA) anchor protein 12 [ Homo sapiens ]
• Official Symbol: AKAP12
• Synonyms: AKAP12; A kinase (PRKA) anchor protein 12; A kinase (PRKA) anchor protein (gravin) 12; A-kinase anchor protein 12; AKAP250; gravin; Src Suppressed C Kinase Substrate; SSeCKS; AKAP 250; kinase scaffold protein gravin; A-kinase anchor protein, 250kDa; Src-Suppressed C Kinase Substrate; myasthenia gravis autoantigen gravin; FLJ20945; FLJ97621; DKFZp686M0430; DKFZp686O0331
• Gene ID: 9590
• mRNA Refseq: NM_005100
• Protein Refseq: NP_005091
• MIM: 604698
• UniProt ID: Q02952
• Chromosome Location: 6q24-q25
• Pathway: G Protein Signaling Pathways, organism-specific biosystem
• Function: adenylate cyclase binding; protein binding; protein kinase A binding