Recombinant Human AGAP3 protein, His-tagged

• Cat.No.: AGAP3-2744H
• Product Overview: Recombinant Human AGAP3 protein(474-556 aa), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: 474-556 aa
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization.
• AASequence: LAIGPCKSLPNSPSHSAVSAASIPA VHINQATNGGGSAFSDYSSSVPSTP SISQRELRIETIAASSTPTPIRKQS KRRSNIFT
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks). Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 μg/μl in 200 μl sterile water for short-term storage. After reconstitution with sterile water, if glycerol has no effect on subsequent experiments, it is recommended to add an equal volume of glycerol for long-term storage.

Gene Information

• Gene Name: AGAP3 ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 [ Homo sapiens ]
• Official Symbol: AGAP3
• Synonyms: AGAP3; ArfGAP with GTPase domain, ankyrin repeat and PH domain 3; centaurin, gamma 3 , CENTG3; arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3; centaurin-gamma-3; centaurin, gamma 3; CRAM-associated GTPase; MR1-interacting protein; CRMP (collapsin response mediator protein) associated; CRAG; AGAP-3; CENTG3; MRIP-1; cnt-g3; FLJ16146; FLJ34452
• Gene ID: 116988
• mRNA Refseq: NM_001042535
• Protein Refseq: NP_001036000
• UniProt ID: Q96P47

Reviews

09/03/2020

The product’s high purity facilitated precise quantification, minimizing variability in our data.

04/08/2020

Agap3's absence of contaminants contributed to data accuracy.

10/14/2018

 The product’s innovative characteristics allowed us to delve into previously unexplored cellular mechanisms.

06/04/2018

The consistent effects of Agap3 streamlined our research process.

01/16/2016

It triggered a robust immune response, evident in cytokine levels.

Q&As

Are there any other proteins similar to AGAP3?

Yes, AGAP3 belongs to a family of Arf GTPase-activating proteins (ArfGAPs) that share similar structural domains.

Is AGAP3 expression tissue-specific?

 Yes, AGAP3 expression can vary between different tissues and cell types.

Where is AGAP3 found?

AGAP3 is primarily located in the cytoplasm of cells.

What is the molecular weight of AGAP3 protein?

The molecular weight of AGAP3 is approximately 150 kDa.

Does AGAP3 play a role in neuronal development?

Yes, AGAP3's involvement in vesicle trafficking is crucial for neuronal development and synaptic function.

What is AGAP3 protein?

AGAP3 is a protein that plays a role in intracellular trafficking and membrane dynamics.

Can AGAP3 influence cancer progression?

AGAP3's involvement in vesicle trafficking and cell migration can contribute to tumor growth and metastasis in certain cancers.

How does AGAP3 interact with Arf GTPases?

AGAP3 acts as a GTPase-activating protein (GAP) for Arf GTPases, regulating their activity in intracellular trafficking pathways.

How does AGAP3 affect endocytosis?

AGAP3 participates in endocytic processes by regulating the formation and trafficking of endocytic vesicles.

How is AGAP3's expression regulated?

AGAP3's expression can be regulated by various factors, including cellular signaling pathways and transcriptional regulators.

What is the PH domain in AGAP3 responsible for?

The PH domain in AGAP3 is thought to be involved in membrane targeting and interaction with phospholipids.

How does AGAP3 impact cell migration?

AGAP3 influences cell migration by regulating the turnover of adhesion complexes and actin dynamics.

What cellular processes does AGAP3 participate in?

AGAP3 is involved in vesicle trafficking, endocytosis, and cell migration.

What is the full name of AGAP3?

AGAP3 stands for Arf-GAP with GTPase domain, Ankyrin repeat, and PH domain 3.

Can AGAP3's role in cellular processes be modified?

Yes, AGAP3's function can be influenced by factors such as post-translational modifications and protein interactions.

What is the function of the Ankyrin repeat in AGAP3?

The Ankyrin repeat in AGAP3 is believed to play a role in protein-protein interactions.

What techniques are used to study AGAP3's function?

Researchers use techniques like molecular biology, microscopy, and cell culture experiments to study AGAP3's role in cells.

How does AGAP3 contribute to vesicle trafficking?

AGAP3 helps regulate the movement of vesicles within cells, aiding in the transportation of molecules.

Are there any known diseases associated with AGAP3 mutations?

Mutations in AGAP3 have been linked to certain neurological disorders and cancer progression.

Can AGAP3 be localized to specific subcellular compartments?

Yes, AGAP3 can be found in various subcellular compartments, including the plasma membrane, endosomes, and Golgi apparatus.

Does AGAP3 play a role in signal transduction?

Yes, AGAP3 can impact signal transduction pathways by regulating membrane trafficking of receptors and signaling molecules.

How is AGAP3's GAP activity activated?

AGAP3's GAP activity is often activated by interactions with activated Arf GTPases, triggering the hydrolysis of GTP to GDP.

What happens when AGAP3 is knocked down or inhibited?

Knocking down or inhibiting AGAP3 can lead to disrupted vesicle trafficking, altered cell adhesion, and changes in cellular morphology.