Recombinant Human ADRA1A protein, His & T7-tagged

• Cat.No.: ADRA1A-6778H
• Product Overview: Recombinant Human ADRA1A aa. (Ser330~Val466 (Accession # P35348)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 19.2kDa.
• Protein length: Ser330~Val466
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: ADRA1A adrenoceptor alpha 1A [ Homo sapiens (human) ]
• Official Symbol: ADRA1A
• Synonyms: ADRA1C; ADRA1L1; ALPHA1AAR; G protein coupled receptor; adrenergic, alpha-1A-, receptor; alpha-1A adrenoceptor; alpha-1A adrenoreceptor; alpha-1C adrenergic receptor; alpha-1A adrenergic receptor
• Gene ID: 148
• mRNA Refseq: NM_000680.3
• Protein Refseq: NP_000671.2
• UniProt ID: P35348