Recombinant Human ADAMTS13 protein, His & GST-tagged

• Cat.No.: ADAMTS13-5661H
• Product Overview: Recombinant Human ADAMTS13 aa. (Val896~Val1131) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Val896~Val1131
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 4.7.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: ADAMTS13 ADAM metallopeptidase with thrombospondin type 1 motif 13 [ Homo sapiens (human) ]
• Official Symbol: ADAMTS13
• Synonyms: ADAMTS13; ADAM metallopeptidase with thrombospondin type 1 motif 13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13; A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13; vWF-cleaving protease; von Willebrand factor-cleaving protease
• Gene ID: 11093
• mRNA Refseq: NM_139025.4
• Protein Refseq: NP_620594.1
• UniProt ID: Q76LX8