Recombinant Human ADAM15 protein, His-tagged

• Cat.No.: ADAM15-1717H
• Product Overview: Recombinant Human ADAM15 aa. (Ser505~Arg745) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ser505~Arg745
• Description: The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Form: Freeze-dried powder
• Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 7.1

Gene Information

• Gene Name: ADAM15 ADAM metallopeptidase domain 15 [ Homo sapiens (human) ]
• Official Symbol: ADAM15
• Synonyms: ADAM15; ADAM metallopeptidase domain 15; MDC15; disintegrin and metalloproteinase domain-containing protein 15; MDC-15; a disintegrin and metalloproteinase domain 15 (metargidin); metalloprotease RGD disintegrin protein; metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15
• Gene ID: 8751
• mRNA Refseq: NM_001261464.1
• Protein Refseq: NP_001248393.1
• UniProt ID: Q13444