Recombinant Human acyl-CoA dehydrogenase family, member 8, His-tagged

• Cat.No.: ACAD8-338H
• Product Overview: ACAD8, 23-415aa, Human, His tag, E.coli

Specification

• Description: ACAD8 is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. The protein is a mitochondrial enzyme that functions in catabolism of the branched-chain amino acid valine. Defects in this gene are the cause of isobutyryl-CoA dehydrogenase deficiency. Recombinant human ACAD8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
• Molecular Mass: 45.1kDa (416aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSLVQTGHR SLTSCIDPSM GLNEEQKEFQ KVAFDFAARE MAPNMAEWDQ KELFPVDVMR KAAQLGFGGV YIQTDVGGSG LSRLDTSVIF EALATGCTST TAYISIHNMC AWMIDSFGNE EQRHKFCPPL CTMEKFASYC LTEPGSGSDA ASLLTSAKKQ GDHYILNGSK AFISGAGESD IYVVMCRTGG PGPKGISCIV VEKGTPGLSF GKKEKKVGWN SQPTRAVIFE DCAVPVANRI GSEGQGFLIA VRGLNGGRIN IASCSLGAAH ASVILTRDHL NVRKQFGEPL ASNQYLQFTL ADMATRLVAA RLMVRNAAVA LQEERKDAVA LCSMAKLFAT DECFAICNQA LQMHGGYGYL KDYAVQQYVR DSRVHQILEG SNEVMRILIS RSLLQE
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)
• Protein length: 23-415 a.a.

Gene Information

• Gene Name: ACAD8 acyl-CoA dehydrogenase family, member 8 [ Homo sapiens ]
• Official Symbol: ACAD8
• Synonyms: ACAD8; acyl-CoA dehydrogenase family, member 8; acyl Coenzyme A dehydrogenase family, member 8; isobutyryl-CoA dehydrogenase, mitochondrial; activator-recruited cofactor 42 kDa component; acyl-Coenzyme A dehydrogenase family, member 8; ARC42; ACAD-8
• Gene ID: 27034
• mRNA Refseq: NM_014384
• Protein Refseq: NP_055199
• MIM: 604773
• UniProt ID: Q9UKU7
• Chromosome Location: 11q25
• Pathway: Branched-chain amino acid catabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Valine, leucine and isoleucine degradation, organism-specific biosystem; Valine, leucine and isoleucine degradation, conserved biosystem; valine degradation I, organism-specific biosystem
• Function: acyl-CoA dehydrogenase activity; acyl-CoA dehydrogenase activity; flavin adenine dinucleotide binding