Recombinant Human ACADSB Protein, His&GST-tagged

• Cat.No.: ACADSB-24H
• Product Overview: Recombinant Human ACADSB Protein (Leu218-Tyr432) with N-His&GST tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Leu218-Tyr432
• Description: Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 53.7 kDa
  Accurate Molecular Mass: 54 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: ACADSB acyl-CoA dehydrogenase, short/branched chain [ Homo sapiens (human) ]
• Official Symbol: ACADSB
• Synonyms: ACADSB; acyl-CoA dehydrogenase, short/branched chain; ACAD7; SBCAD; 2-MEBCAD; short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; 2-methylbutyryl-coenzyme A dehydrogenase; 2-methyl branched chain acyl-CoA dehydrogenase; 2 MEBCAD; 2 methyl branched chain acyl CoA dehydrogenase; 2 methylbutyryl CoA dehydrogenase; 2-methylbutyryl-CoA dehydrogenase; ACDSB_HUMAN; acyl CoA dehydrogenase, short/branched chain; OTTHUMP00000020685; OTTHUMP00000046795; Short/branched chain specific acyl-CoA dehydrogenase; acyl-Coenzyme A dehydrogenase, short/branched chain; 2-methylbutyryl-coenzyme A dehydrogenase; short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; EC 1.3.99
• Gene ID: 36
• mRNA Refseq: NM_001609
• Protein Refseq: NP_001600
• MIM: 600301
• UniProt ID: P45954