Recombinant Human ACADSB 293 Cell Lysate

• Cat.No.: ACADSB-9112HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACADSB acyl-CoA dehydrogenase, short/branched chain [ Homo sapiens ]
• Official Symbol: ACADSB
• Synonyms: ACADSB; acyl-CoA dehydrogenase, short/branched chain; ACAD7; SBCAD; 2-MEBCAD; short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; 2-methylbutyryl-coenzyme A dehydrogenase; 2-methyl branched chain acyl-CoA dehydrogenase; 2 MEBCAD; 2 methyl branched chain acyl CoA dehydrogenase; 2 methylbutyryl CoA dehydrogenase; 2-methylbutyryl-CoA dehydrogenase; ACDSB_HUMAN; acyl CoA dehydrogenase, short/branched chain; OTTHUMP00000020685; OTTHUMP00000046795; Short/branched chain specific acyl-CoA dehydrogenase; acyl-Coenzyme A dehydrogenase, short/branched chain; 2-methylbutyryl-coenzyme A dehydrogenase; short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; EC 1.3.99
• Gene ID: 36
• mRNA Refseq: NM_001609
• Protein Refseq: NP_001600
• MIM: 600301
• UniProt ID: P45954
• Chromosome Location: 10q26.13
• Pathway: Branched-chain amino acid catabolism, organism-specific biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Valine, leucine and isoleucine degradation, organism-specific biosystem; Valine, leucine and isoleucine degradation, conserved biosystem
• Function: acyl-CoA dehydrogenase activity; electron carrier activity; flavin adenine dinucleotide binding; short-branched-chain-acyl-CoA dehydrogenase activity