Recombinant Human AATF 293 Cell Lysate
Cat.No. : | AATF-9155HCL |
- Specification
- Gene Information
- Related Products
- Download
Species : | Human |
Source : | HEK293 |
Tag : | Non |
Description : | Antigen standard for apoptosis antagonizing transcription factor (AATF) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name | AATF apoptosis antagonizing transcription factor [ Homo sapiens ] |
Official Symbol | AATF |
Synonyms | AATF; apoptosis antagonizing transcription factor; protein AATF; CHE 1; CHE1; DED; rb-binding protein Che-1; apoptosis-antagonizing transcription factor; CHE-1; |
Gene ID | 26574 |
mRNA Refseq | NM_012138 |
Protein Refseq | NP_036270 |
MIM | 608463 |
UniProt ID | Q9NY61 |
Chromosome Location | 17q12 |
Pathway | Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; NRAGE signals death through JNK, organism-specific biosystem; Regulation of retinoblastoma protein, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signalling by NGF, organism-specific biosystem; p75 NTR receptor-mediated signalling, organism-specific biosystem; |
Function | leucine zipper domain binding; protein binding; protein domain specific binding; sequence-specific DNA binding transcription factor activity; tau protein binding; |
◆ Recombinant Proteins | ||
PACSIN1-1506H | Recombinant Human PACSIN1, GST-tagged | +Inquiry |
HGD-9432Z | Recombinant Zebrafish HGD | +Inquiry |
GPN1-13429H | Recombinant Human GPN1, GST-tagged | +Inquiry |
RFL1304CF | Recombinant Full Length Corynebacterium Glutamicum Upf0059 Membrane Protein Cgl1469/Cg1660(Cgl1469, Cg1660) Protein, His-Tagged | +Inquiry |
ACE2-185H | Active Recombinant Human ACE2 protein, mFc-tagged | +Inquiry |
◆ Native Proteins | ||
FABP-177R | Native Rabbit Fatty acid Binding Protein | +Inquiry |
IgG1-227H | Native Human Immunoglobulin G1 (IgG1) | +Inquiry |
MOG-20 | Native Mouse/Rat MOG (35-55) Protein | +Inquiry |
Testosterone-01H | Native Human Testosterone | +Inquiry |
CDA002 | Active Native Human MUC16 Protein | +Inquiry |
◆ Cell & Tissue Lysates | ||
PAX4-3417HCL | Recombinant Human PAX4 293 Cell Lysate | +Inquiry |
MB21D2-8040HCL | Recombinant Human C3orf59 293 Cell Lysate | +Inquiry |
C16orf72-8246HCL | Recombinant Human C16orf72 293 Cell Lysate | +Inquiry |
Ileum-245R | Rhesus monkey Ileum Lysate | +Inquiry |
GDF10-2101MCL | Recombinant Mouse GDF10 cell lysate | +Inquiry |
Not For Human Consumption!
Inquiry
- Reviews
- Q&As
Ask a Question for All AATF Products
Required fields are marked with *
My Review for All AATF Products
Required fields are marked with *
0
Inquiry Basket