Recombinant Human A2M Protein, N-His-tagged

• Cat.No.: A2M-13H
• Product Overview: Recombinant Human A2M Protein, fused to His-tag at N-terminus, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4).
• Molecular Mass: 160 kDa
• Endotoxin: <0.5 EU per µg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE under reducing conditions and visualized by Coomassie blue staining.
• Usage: This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
• Storage: Upon arrival, the protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store at -20 centigrade or -80 centigrade in appropriate aliquots. Avoid repeated freeze-thaw cycles.
• Concentration: Determined by BCA protein assay.
• Reconstitution: Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
• Shipping: Ice packs

Gene Information

• Gene Name: A2M alpha-2-macroglobulin [ Homo sapiens (human) ]
• Official Symbol: A2M
• Synonyms: A2MD; CPAMD5; FWP007; S863-7
• Gene ID: 2
• mRNA Refseq: NM_000014.6
• Protein Refseq: NP_000005.3
• MIM: 103950
• UniProt ID: P01023