Recombinant Human 5',3'-Nucleotidase, Mitochondrial, His-tagged

• Cat.No.: NT5M-1722H
• Product Overview: Recombinant humanNT5M protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: NT5M, also known asDNT2, belongs to the 5"(3")-deoxyribonucleotidase family. It localizes to themitochondrial matrix. This enzyme dephosphorylates the 5"- and2"(3")-phosphates of uracil and thymine deoxyribonucleotides. The gene islocated on chromosome 17 p11.2 in the critical region deleted in theSmith-Magenis syndrome (SMS), a genetic disease of unknown etiology.
• Concentration: 0.5 mg/ml
• Form: Supplied as a liquidin 20 mM Tris-HCl buffer, pH 8.0, with 20% glycerol, 0.1 M NaCl and 1 mM DTT.
• Purity: > 98% pure bySDS-PAGE
• Sequence: 32-228 amino acids:MGSSHHHHHH SSGLVPRGSH MGGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQYGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKYFGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKAILDS
• Molecular Mass: 25.1 kDa
• Applications: SDS-PAGE
• Storage: Store at 4 deg C forshort term storage. Aliquot and store at -20 deg C or -70 deg C for long termstorage. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: NT5M
• nullValue_other: Q9NPB1

Gene Information

• Gene Name: NT5M 5",3"-nucleotidase,mitochondrial [ Homo sapiens ]
• Synonyms: NT5M;5",3"-nucleotidase, mitochondrial; mdN; dNT2; dNT-2;5"(3")-deoxyribonucleotidase, mitochondrial; OTTHUMP00000065515;deoxy-5"-nucleotidase 2; 5(3)-deoxyribonucleotidase; mitochondrial 5"nucleotidase; 5" nucleotidase, mitochondrial; EC 3.1.3.5
• Gene ID: 56953
• mRNA Refseq: NM_020201
• Protein Refseq: NP_064586
• MIM: 605292
• Chromosome Location: 17p11.2
• Pathway: Metabolic pathways;Metabolism of nucleotides; Nicotinate and nicotinamide metabolism; Purinemetabolism; Pyrimidine catabolism; Pyrimidine metabolism
• Function: phosphataseactivity; nucleotide binding; nucleotidase activity; metal ion binding;hydrolase activity; 5"-nucleotidase activity