Recombinant Human 3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase, His-tagged

• Cat.No.: HMGCL-1562H
• Product Overview: Recombinant human HMGCL protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: HMGCL, also known as hydroxymethylglutaryl-CoA lyase, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It exists as a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies that, during fasting, provide a major source of energy for heart, brain and kidney. More specifically, it catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea.
• Molecular Weight: 34.2 kDa (323aa)
• Purity: >85% by SDS-PAGE
• Concentration: 1.0 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMTLPKR VKIVEVGPRD GLQNEKNIVS TPVKIKLIDM LSEAGLSVIE TTSFVSPKWV PQMGDHTEVLKGIQKFPGIN YPVLTPNLKG FEAAVAAGAK EVVIFGAASE LFTKKNINCS IEESFQRFDA ILKAAQSANI SVRGYVSCAL GCPYEGKISPAKVAEVTKKF YSMGCYEISL GDTIGVGTPG IMKDMLSAVM QEVPLAALAV HCHDTYGQAL ANTLMALQMG VSVVDSSVAG LGGCPYAQGASGNLATEDLV YMLEGLGIHT GVNLQKLLEA GNFICQALNR KTSSKVAQAT CKL
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Butanoate metabolism; Fatty acid; Formation of Acetoacetic Acid; Ketone body metabolism; Metabolic pathways; Metabolism of lipids and lipoproteins; Peroxisome; Synthesis and Degradation of Ketone Bodies; Synthesis of Ketone Bodies; Valine

Gene Information

• Gene Name: HMGCL 3-hydroxymethyl-3-methylglutaryl-CoA lyase [ Homo sapiens ]
• Official Symbol: HMGCL
• Synonyms: HMGCL; 3-hydroxymethyl-3-methylglutaryl-CoA lyase; HL; hydroxymethylglutaryl-CoA lyase, mitochondrial; HMG-CoA lyase; OTTHUMP00000003005; OTTHUMP00000223529; OTTHUMP00000223530; EC 4.1.3.4; hydroxymethylglutaricaciduria; 3-hydroxy-3-methylglutaryl-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
• Gene ID: 3155
• mRNA Refseq: NM_000191
• Protein Refseq: NP_000182
• MIM: 613898
• UniProt ID: P35914
• Chromosome Location: 1p36.1-p35
• Function: carboxylic acid binding; fatty-acyl-CoA binding; hydroxymethylglutaryl-CoA lyase activity; lyase activity; metal ion binding