Recombinant HIV nef protein

• Cat.No.: nef-177H
• Product Overview: The E.coli derived 27kDa recombinant HIV-1 Nef Clade-B protein is a single non-glycosylated polypeptide chain.

Specification

• Species: HIV
• Source: E.coli
• Tag: Non
• Description: HIV-1 Nef is a 27kDa protein highly produced after a very short time after virus infection. HIV-1 Nef is an essential factor for efficient viral replication and phatogenesis.HIV-1 Nef facilitates virus replication and improves virions infectivity. HIV-1 Nef exerts pleiotropic effect. HIV-1 Nef down-modulates surface MHC-I molecules, decreases cell surface CD4 antigen by interacting with the Src family kinase LCK thereby inducing LCK-CD4 dissociation and by increasing clathrindependent endocytosis of this antigen to target it to lysosomial degradation. HIV-1 Nef guards the infected cell from apoptosis in order to keep it alive until the next virus generation is ready to strike. HIV-1 Nef protein bypasses host Tcell signaling by inducing a transcriptional program nearly identical to that of anti-CD3 cell activation.
• Form: Sterile Filtered and lyophilized, though might appear as a solution as a result of the glycerol content. Lyophilized with 10% glycerol.
• Molecular Mass: 27kDa
• Purity: Greater than 90.0% as determined by HPLC analysis and SDS-PAGE.
• Usage: The products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
• Stability: Lyophilized HIV-1 nef although stable at room temperature for 1 week, should be stored desiccated below -18°C. Upon reconstitution HIV-1 nef should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Solubility: It is recommended to reconstitute the lyophilized HIV-1 nef in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: nef p27 [ Human immunodeficiency virus 1 ]
• Official Symbol: nef
• Synonyms: nef; p27
• Gene ID: 156110
• Protein Refseq: NP_057857.2
• UniProt ID: P04601