Recombinant HIV-1(group M, subtype CRF07_BC) gp120 protein(Ala29-Glu502), His-tagged

• Cat.No.: gp120-1091V
• Product Overview: Recombinant HIV-1 envelope glycoprotein gp120 (AFM77980.1) (Ala29-Glu502) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: HIV
• Source: HEK293
• Tag: His
• Protein Length: Ala29-Glu502
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant HIV-1 gp120 consists of 485 amino acids after removal of the signal peptide and has a predicted molecular mass of 54.8 kDa. The apparent molecular mass of the recombinant protein is approximately 70-120 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.