Recombinant HIV-1(group M, subtype B, Isolate MN) gp120 protein(Thr30-Arg513), His-tagged

• Cat.No.: gp120-1122V
• Product Overview: Recombinant HIV1(group M, subtype B, isolate MN) Envelope glycoprotein gp160 Protein(gp120 subunit) (AAC31819.1) (Thr30-Arg513) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: HIV
• Source: HEK293
• Tag: His
• ProteinLength: Thr30-Arg513
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant HIV1(group M, subtype B, isolate MN) Envelope glycoprotein gp160 Protein(gp120 subunit) consists of 495 amino acids and predicts a molecular mass of 55.8 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.