Recombinant HIV-1 (group M, subtype B, strain SHIV-89.6P) gp120 Protein, His-tagged

• Cat.No.: Gp120-545H
• Product Overview: Recombinant HIV-1 (group M, subtype B, strain SHIV-89.6P) gp120 Protein(AAC57427.1)(Thr31-Arg509), fused with His tag, was expressed in HEK293.

Specification

• Species: HIV
• Source: HEK293
• Tag: His
• Protein Length: Thr31-Arg509
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant Human immunodeficiency virus 1(group M, subtype B, strain SHIV-89.6P) ENV consists 490 amino acids and predicts a molecular mass of 55.4 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.