Recombinant HIV-1 (group M, subtype A, isolate 92RW020) gp120 protein(Glu31-Arg494), His-tagged

• Cat.No.: gp120-3321H
• Product Overview: Recombinant HIV-1 envelope glycoprotein gp160 (AAT67478.1)(Glu31-Arg494),termed as gp120, was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: HIV
• Source: HEK293
• Tag: His
• ProteinLength: Glu31-Arg494
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant HIV-1 envelope glycoprotein gp160 consists 465 amino acids and predicts a molecular mass of 52.2 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.